{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE269nnn/GSE269288/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Gallus gallus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE269288"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Dynamics of Immortalized Chicken Fibroblasts from Adherent Cultivation to Carrier-Free Suspension Culture","description":"This study aimed to elucidate the internal mechanisms of biological changes in immortalized chicken embryo fibroblasts (DF-1) by transitioning them from adherent to suspension culture. Initially, we determined the optimal growth conditions necessary for suspension cell culture. The adherent DF-1 cells (2D) were then adapted to suspension culture, resulting in short-term (3D_E) and long-term (3D_L) suspension cells. Growth metrics were monitored throughout the suspension phase. Subsequently, RNA-seq analysis was conducted to identify key genes and signaling pathways that exhibited distinct changes in 2D, 3D_E and 3D_L cells. The transcriptome data guided further investigations into the transdifferentiation of 2D, 3D_E, and 3D_L cells into lipids and their collagen secretion capabilities.","dates":{"publication":"2026/07/01"},"accession":"GSE269288","cross_references":{"GSM":["GSM8312443","GSM8312444","GSM8312441","GSM8312442","GSM8312440","GSM8312438","GSM8312439","GSM8312436","GSM8312437"],"GPL":["24996"],"GSE":["269288"],"taxon":["Gallus gallus"]}}