GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE269nnn/GSE269288/primary200OKTranscriptomicsGallus gallusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE269288GEOGSEfalseMolecular Dynamics of Immortalized Chicken Fibroblasts from Adherent Cultivation to Carrier-Free Suspension CultureThis study aimed to elucidate the internal mechanisms of biological changes in immortalized chicken embryo fibroblasts (DF-1) by transitioning them from adherent to suspension culture. Initially, we determined the optimal growth conditions necessary for suspension cell culture. The adherent DF-1 cells (2D) were then adapted to suspension culture, resulting in short-term (3D_E) and long-term (3D_L) suspension cells. Growth metrics were monitored throughout the suspension phase. Subsequently, RNA-seq analysis was conducted to identify key genes and signaling pathways that exhibited distinct changes in 2D, 3D_E and 3D_L cells. The transcriptome data guided further investigations into the transdifferentiation of 2D, 3D_E, and 3D_L cells into lipids and their collagen secretion capabilities.2026/07/01GSE269288GSM8312443GSM8312444GSM8312441GSM8312442GSM8312440GSM8312438GSM8312439GSM8312436GSM831243724996269288Gallus gallus