{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE269nnn/GSE269909/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE269909"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Divergent medulloblastoma chromatin states disclose KDM2B as a selective dependency [RNA-Seq]","description":"Medulloblastoma (MB) is a biologically heterogeneous childhood cerebellar tumor harboring frequent chromatin-modifying gene alterations. How these alterations promote transcriptional programs governing malignancy remains poorly defined. To address this knowledge gap, we evaluated chromatin states across MB subgroups by multi-modal integration of histone modifications with mutational, DNA methylation, and transcriptomic profiles. A bivalent/poised enhancer (EnhBiv) state was specifically enriched at the promoters of neurodevelopmental genes in Groups 3 and 4-MB. Integrative bioinformatics coupled with chromatin occupancy studies identified aberrant KDM2B binding at EnhBiv-enriched promoters. CRISPR-mediated knockout or acute protein degradation of KDM2B selectively suppressed the growth of MB models in vitro and in vivo. Mechanistically, KDM2B promotes sequential recruitment of Polycomb repressive complexes (PRC1/PRC2) and EnhBiv chromatin, thereby repressing neuronal differentiation programs. Collectively, we provide foundational insights into an epigenetic basis of MB, nominating KDM2B as a selective dependency in high-risk subgroups that warrants consideration as a candidate therapeutic target.","dates":{"publication":"2026/06/26"},"accession":"GSE269909","cross_references":{"GSM":["GSM9570165","GSM9570166","GSM9570163","GSM9570164","GSM8330120","GSM9570169","GSM8330121","GSM9570167","GSM9570168","GSM8330124","GSM8330125","GSM8330122","GSM8330123","GSM8330128","GSM9570161","GSM8330129","GSM9570162","GSM8330126","GSM9570160","GSM8330127","GSM8330119","GSM9570176","GSM9570174","GSM9570175","GSM8330131","GSM8330132","GSM8330130","GSM8330135","GSM8330136","GSM8330133","GSM8330134","GSM9570172","GSM8330139","GSM9570173","GSM8330137","GSM9570170","GSM9570171","GSM8330138","GSM8330142","GSM8330140","GSM8330141","GSM9570159","GSM8330113","GSM8330114","GSM8330111","GSM8330112","GSM8330117","GSM8330118","GSM8330115","GSM8330116"],"GPL":["24676"],"GSE":["269909"],"taxon":["Homo sapiens"]}}