{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE271nnn/GSE271440/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE271440"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Robotic Generation and Kinetic Screening of Patient-Derived Glioblastoma Organoids Reveal Pharmaceutical Dynamics","description":"Glioblastoma (GBM) presents a significant challenge in oncology due to its high heterogeneity and poor response to conventional therapies. Current preclinical models are highly variable, creating disparate patient-derived organoids. We developed a Robot-Directed Organoid Deposition (RODEO) system that reproducibly generates complex patient-derived GBM organoids for high-throughput screening. RODEO-GBM organoids recapitulate the cellular heterogeneity, specific tumor niches, and complex vascularization of GBM. Integrating tissue-embedded microsensors in RODEO-GBM organoids allowed us to screen 126 FDA-approved oncology drugs, on 1,638 patient-derived organoids, generating 105 data points in 96 hrs. Our screen revealed distinct kinetic patterns of drug responses, identifying transient as well as slow-acting compounds often overlooked by conventional methods. Slow-acting drugs preferentially targeted stem-like SOX2+ tumor cells, thus preserving peripheral cell populations. Comparative analysis on RODEO-Liver organoids showed that slow-acting drugs exhibit lower liver toxicity supporting a higher therapeutic index. Coupling RODEO with sensor-based kinetic screening provides critical new insights into pharmaceutical dynamics, paving the way for the identification of more effective treatments for GBM and other cancers.","dates":{"publication":"2026/03/31"},"accession":"GSE271440","cross_references":{"GSM":["GSM8376965","GSM8376967","GSM8376966","GSM8376968"],"GPL":["30173"],"GSE":["271440"],"taxon":["Homo sapiens"]}}