{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE271nnn/GSE271663/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE271663"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Tumor-Suppressing Multi-Enterobacteria enhance the anti-PD-1/PD-L1 efficacy in microsatellite stable colorectal cancer","description":"Gut microbiome significantly influences immunotherapy responses in colorectal cancer (CRC) treatment. While individual enterobacteria have been identified as enhancers of anti-PD-1/anti-PD-L1 therapy, the synergistic effects of multiple enterobacteria remain underexplored. To fill the gap, we introduced Tumor-Suppressing Multi-Enterobacteria (TSME), a consortium of nine beneficial intestinal probiotic strains, and investigated its impact on the anti-PD-1/anti-PD-L1 therapy for microsatellite stable (MSS) CRC. Using a tumor-bearing mouse model and genomic sequencing techniques, our research demonstrated that TSME significantly improved therapy efficacy by optimizing tumor immune microenvironment. Specifically, the addition of TSME notably increased CD8+ T infiltration, modulated cytokine profiles, and up-regulated crucial immune-related pathways, including TNF and JAK-STAT. Additionally, TSME altered intestinal microbial composition, enriching beneficial bacteria such as Akkermansia and Alistipes. These findings suggest that the well-engineered multi-enterobacteria could significantly enhance the effectiveness of immunotherapy for MSS CRC by synergistically modulating the immune and microbial landscapes.","dates":{"publication":"2026/04/26"},"accession":"GSE271663","cross_references":{"GSM":["GSM8381394","GSM8381393","GSM8381374","GSM8381396","GSM8381395","GSM8381398","GSM8381376","GSM8381397","GSM8381375","GSM8381378","GSM8381377","GSM8381399","GSM8381390","GSM8381392","GSM8381391","GSM8381409","GSM8381402","GSM8381401","GSM8381404","GSM8381403","GSM8381406","GSM8381405","GSM8381408","GSM8381407","GSM8381383","GSM8381382","GSM8381385","GSM8381384","GSM8381387","GSM8381386","GSM8381400","GSM8381389","GSM8381388","GSM8381381","GSM8381380","GSM8381379"],"GPL":["24247"],"GSE":["271663"],"taxon":["Mus musculus"]}}