GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE272nnn/GSE272953/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE272953GEOGSEfalseMDA5 regulates bleomycin-induced proinflammatory response of macrophagesThe role of innate immune receptors recognizing RNA and self RNA in lung inflammation and fibrosis formation is not well understood. Here, we demonstrate melanoma differentiation-associated gene 5 (MDA5) regulates bleomycin-induced lung fibrosis, accompanied by increased IL-17+ γδ T cells and neutrophils compared to wild-type (WT) mice. Lung CD11b+ macrophages, with similar characteristics of human Spp1+ macrophages, displayed unique gene expression profiles, marked by elevated levels of ligands for CCR2 and collagen production. Moreover, MDA5 was found to regulate these ligands in bleomycin-treated bone marrow-derived macrophages and U937 cells, contributing to the clearance of extracellular RNA. In conclusion, our findings suggest that MDA5 plays a pivotal role in regulating bleomycin-induced lung fibrosis through influencing the interplay among macrophages, lymphocytes, and neutrophils.2026/07/24GSE272953GSM8415519GSM8415527GSM8415526GSM8415518GSM8415529GSM8415528GSM8415523GSM8415522GSM8415525GSM8415524GSM8415521GSM841552024247272953Mus musculus