GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE27nnn/GSE27314/primaryOK2000000GenomicsDanio rerioGenome binding/occupancy profiling by genome tiling arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27314GEOGSE0falseMarking of the genome by H3K4 methylation prior to embryonic gene activationEarly zebrafish embryo development proceeds first from a maternally transcribed and stored mRNAs, and zygotic gene activation (ZGA) is initiated at the mid-blastula transition (MBT; 1000-cell stage), 3.3 h post-fertilization. Very little is known on how the zygotic genome is programmed for transcriptional activation at the MBT. To start addressing this issue, we have mapped by ChIP-chip genome-wide promoter histone methylation (H3K4me3, H3K9me3, H3K27me3, H3K36me3) and RNA Pol II profiles before ZGA (256-cell stage; 2.5 hpf), during ZGA (MBT; 3.5 hpf)) and after ZGA (Post-MBT; 5.3 hpf) . We used a custom 2.1M probe HD promoter array (Nimblegen) for ChIP and input DNA hybridization. Peak detection was done using MA2C with P=10e-4 as cutoff.2011/12/02GSE27314GSM675190GSM675192GSM675191GSM675194GSM675193GSM675174GSM675196GSM675195GSM675176GSM675198GSM675197GSM675175GSM675178GSM675177GSM675179GSM675181GSM675180GSM675183GSM675182GSM675185GSM675184GSM675187GSM675186GSM675189GSM6751881083527314Danio rerio[22137762]