{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE274nnn/GSE274125/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE274125"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Timestamp tracing delineates thymic regulatory T cell ontogeny in native conditions [scRNA-seq]","description":"Thymic T-cell selection and regulatory T (Treg) cell development are executed via mechanisms that remain unclear. Here, we develop a timestamp tracing method using Rag1CreER knockin mice in combination with Rosa reporters to reveal the processes governing thymic Treg differentiation in native physiological conditions. It enables tracing of thymic T cells at the double negative and double positive stages upon tamoxifen administration. Following labeled cells over time, we define the processes, kinetics, and gene expression changes during thymic T-cell differentiation; specify thymic Treg maturation with CD73, gene expression changes, and Treg-specific DNA demethylation; characterize precursor cells with 4-1BB and/or CD25 expression that are induced by TCR signaling and differentiate to Treg cells in vitro and in vivo; and determine the features of recirculating or retained mature Treg cells. Thus, Rag1CreER–based timestamp tracing delineates thymic Treg ontogeny, proving the concept for exploring T-cell development and heterogeneity with age-resolved precision.","dates":{"publication":"2026/07/21"},"accession":"GSE274125","cross_references":{"GSM":["GSM8443923","GSM8443924","GSM8443921","GSM8443932","GSM8443933","GSM8443922","GSM8443930","GSM8443931","GSM8443920","GSM8443918","GSM8443929","GSM8443919","GSM8443916","GSM8443927","GSM8443928","GSM8443917","GSM8443925","GSM8443914","GSM8443915","GSM8443926"],"GPL":["19057"],"GSE":["274125"],"taxon":["Mus musculus"],"PMID":["[42533592]"]}}