{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE274nnn/GSE274126/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE274126"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Timestamp tracing delineates thymic regulatory T cell ontogeny in native conditions [RNA-seq]","description":"Thymic T-cell selection and regulatory T (Treg) cell development are executed via mechanisms that remain unclear. Here, we develop a timestamp tracing method using Rag1CreER knockin mice in combination with Rosa reporters to reveal the processes governing thymic Treg differentiation in native physiological conditions. It enables tracing of thymic T cells at the double negative and double positive stages upon tamoxifen administration. Following labeled cells over time, we define the processes, kinetics, and gene expression changes during thymic T-cell differentiation; specify thymic Treg maturation with CD73, gene expression changes, and Treg-specific DNA demethylation; characterize precursor cells with 4-1BB and/or CD25 expression that are induced by TCR signaling and differentiate to Treg cells in vitro and in vivo; and determine the features of recirculating or retained mature Treg cells. Thus, Rag1CreER–based timestamp tracing delineates thymic Treg ontogeny, proving the concept for exploring T-cell development and heterogeneity with age-resolved precision.","dates":{"publication":"2026/07/21"},"accession":"GSE274126","cross_references":{"GSM":["GSM8443938","GSM8443939","GSM8443936","GSM8443937","GSM8443934","GSM8443945","GSM8443935","GSM8443943","GSM8443944","GSM8443941","GSM8443942","GSM8443940"],"GPL":["17021"],"GSE":["274126"],"taxon":["Mus musculus"],"PMID":["[42533592]"]}}