GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE274nnn/GSE274617/primaryOK200OtherMus musculus synthetic constructOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE274617GEOGSEfalseSingle cell transcriptomes of pancreatic pre-invasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity [MERFISH]In this project, we utilized single-cell RNA sequencing (scRNA-seq) and MERFISH spatial transcriptomics to profile the development of pancreatic cancer in a mouse model, tracing its progression from the pre-invasive stage to full malignancy. By employing a reporter gene, we were able to dissect the heterogeneity of metaplastic acinar cells, identifying and profiling six distinct types and states of these cells. We validated their localization within pre-invasive lesions, assessed the heterogeneity across various lesions, and calculated the colocalization of different acinar metaplastic cells with stromal cells.2026/05/01GSE274617GSM845370031217274617Mus musculus synthetic construct