{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275425/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Rattus norvegicus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275425"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Injectable Nerve-Selective Cold Neurolysis for Pain Management: Sensory Neuron Modulation and Clinical Translation","description":"Long-lasting, non-opioid therapies are urgently needed for safe and effective pain management. We previously demonstrated that selective cold neurolysis (SCN) of the sciatic nerve, with ice slurry, leads to long-lasting analgesia in rats. To decipher the molecular signatures of analgesia, rat dorsal root ganglia (DRG) were analyzed using RNA-seq after SCN. SCN induced an upregulation of regeneration-associated genes and concomitant changes in genes related to neuronal excitability. We explored the clinical translational potential of SCN through a single-arm, first-in-human pilot investigation involving 16 patients with chronic pain secondary to knee osteoarthritis. These results show that SCN is safe and tolerable, and associated with a 4-point reduction (p < 0.001) in pain levels, assessed through self-reported Numeric Rating Scores, which peaked at 7 days and persisted for up to 12 weeks after a single injection. These results suggest that SCN orchestrates an analgesic response accompanied by transcriptional changes in the DRG.","dates":{"publication":"2026/08/21"},"accession":"GSE275425","cross_references":{"GSM":["GSM8539430","GSM8539431","GSM8539432","GSM8476691","GSM8539433","GSM8476692","GSM8539434","GSM8539435","GSM8476690","GSM8539436","GSM8539437","GSM8476695","GSM8539438","GSM8476696","GSM8476693","GSM8539439","GSM8476694","GSM8476699","GSM8476697","GSM8476698","GSM8476679","GSM8539440","GSM8539441","GSM8539442","GSM8539443","GSM8539444","GSM8476680","GSM8476681","GSM8539445","GSM8539446","GSM8476684","GSM8476685","GSM8476682","GSM8539429","GSM8476683","GSM8476688","GSM8476700","GSM8476689","GSM8476686","GSM8476687","GSM8476701","GSM8476702"],"GPL":["22396"],"GSE":["275425"],"taxon":["Rattus norvegicus"]}}