GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275425/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275425GEOGSEfalseInjectable Nerve-Selective Cold Neurolysis for Pain Management: Sensory Neuron Modulation and Clinical TranslationLong-lasting, non-opioid therapies are urgently needed for safe and effective pain management. We previously demonstrated that selective cold neurolysis (SCN) of the sciatic nerve, with ice slurry, leads to long-lasting analgesia in rats. To decipher the molecular signatures of analgesia, rat dorsal root ganglia (DRG) were analyzed using RNA-seq after SCN. SCN induced an upregulation of regeneration-associated genes and concomitant changes in genes related to neuronal excitability. We explored the clinical translational potential of SCN through a single-arm, first-in-human pilot investigation involving 16 patients with chronic pain secondary to knee osteoarthritis. These results show that SCN is safe and tolerable, and associated with a 4-point reduction (p < 0.001) in pain levels, assessed through self-reported Numeric Rating Scores, which peaked at 7 days and persisted for up to 12 weeks after a single injection. These results suggest that SCN orchestrates an analgesic response accompanied by transcriptional changes in the DRG.2026/08/21GSE275425GSM8539430GSM8539431GSM8539432GSM8476691GSM8539433GSM8476692GSM8539434GSM8539435GSM8476690GSM8539436GSM8539437GSM8476695GSM8539438GSM8476696GSM8476693GSM8539439GSM8476694GSM8476699GSM8476697GSM8476698GSM8476679GSM8539440GSM8539441GSM8539442GSM8539443GSM8539444GSM8476680GSM8476681GSM8539445GSM8539446GSM8476684GSM8476685GSM8476682GSM8539429GSM8476683GSM8476688GSM8476700GSM8476689GSM8476686GSM8476687GSM8476701GSM847670222396275425Rattus norvegicus