{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275651/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275651"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Dispensable role of RPB1 CTD in co-transcriptional splicing in mouse ESC (Fraction chrRNA-seq)","description":"To define the function of CTD on co-transcriptional splicing, we used auxin-inducible CTD degron system in mouse embryonic stem cells (mESCs) with auxin treatment for 3 hours to sufficiently deplete Rpb9. To measure co-transcriptional splicing, we fractionated mESCs cells (spiked in with Drosophila S2 cells for data normalization) into cytosolic (Cyt), nucleoplasm (NP), and chromatin/particle (Chr) fractions. We purified total RNA from Chr compartment, depleted ribosomal RNAs, and constructed two independent libraries for RNA-seq, which showed excellent reproducibility.","dates":{"publication":"2026/05/04"},"accession":"GSE275651","cross_references":{"GSM":["GSM8481950","GSM8481949","GSM8481947","GSM8481948"],"GPL":["24247"],"GSE":["275651"],"taxon":["Mus musculus"],"PMID":["[25772140]"]}}