GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275651/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275651GEOGSEfalseDispensable role of RPB1 CTD in co-transcriptional splicing in mouse ESC (Fraction chrRNA-seq)To define the function of CTD on co-transcriptional splicing, we used auxin-inducible CTD degron system in mouse embryonic stem cells (mESCs) with auxin treatment for 3 hours to sufficiently deplete Rpb9. To measure co-transcriptional splicing, we fractionated mESCs cells (spiked in with Drosophila S2 cells for data normalization) into cytosolic (Cyt), nucleoplasm (NP), and chromatin/particle (Chr) fractions. We purified total RNA from Chr compartment, depleted ribosomal RNAs, and constructed two independent libraries for RNA-seq, which showed excellent reproducibility.2026/05/04GSE275651GSM8481950GSM8481949GSM8481947GSM848194824247275651Mus musculus[25772140]