{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275756/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275756"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional identity of murine healthy spleenic, tumor-bearing splenic and tumoral Treg cells.","description":"CRC-organoids were implanted in the liver of Foxp3eGFP mice to investigate the phenotypic differences of TI-Treg cells in vivo dependent on location. After tumor development, T cells from distinct metastatic sites were isolated and analyzed. RNA sequencing of Treg cells from the spleen, \"primary\" liver tumors, and metastases revealed that TI-Treg cells expressed core genes previously associated with CRC-related Treg cells, with subtle location-specific variations. Gene Ontology analysis highlighted enriched immune response regulation pathways in primary liver TI-Treg cells compared to healthy splenic Treg cells. High expression of genes upregulated in liver TI-Treg cells was associated with poor CRC prognosis. Splenic Treg cells from tumor-bearing mice also displayed distinct transcriptional profiles from their healthy counterparts. These findings highlight that TI-Treg cells have transcriptional profiles linked to poor CRC prognosis, with subtle location-specific variations.","dates":{"publication":"2026/08/20"},"accession":"GSE275756","cross_references":{"GSM":["GSM8483999","GSM8484009","GSM8483998","GSM8484019","GSM8484018","GSM8484017","GSM8484016","GSM8484015","GSM8484014","GSM8484013","GSM8484012","GSM8484011","GSM8484010","GSM8483989","GSM8483988","GSM8484008","GSM8483997","GSM8484007","GSM8483996","GSM8484006","GSM8483995","GSM8484005","GSM8483994","GSM8483993","GSM8484004","GSM8484003","GSM8483992","GSM8484002","GSM8483991","GSM8483990","GSM8484001","GSM8484023","GSM8484022","GSM8484000","GSM8484021","GSM8484020"],"GPL":["19057"],"GSE":["275756"],"taxon":["Mus musculus"]}}