GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275756/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275756GEOGSEfalseTranscriptional identity of murine healthy spleenic, tumor-bearing splenic and tumoral Treg cells.CRC-organoids were implanted in the liver of Foxp3eGFP mice to investigate the phenotypic differences of TI-Treg cells in vivo dependent on location. After tumor development, T cells from distinct metastatic sites were isolated and analyzed. RNA sequencing of Treg cells from the spleen, "primary" liver tumors, and metastases revealed that TI-Treg cells expressed core genes previously associated with CRC-related Treg cells, with subtle location-specific variations. Gene Ontology analysis highlighted enriched immune response regulation pathways in primary liver TI-Treg cells compared to healthy splenic Treg cells. High expression of genes upregulated in liver TI-Treg cells was associated with poor CRC prognosis. Splenic Treg cells from tumor-bearing mice also displayed distinct transcriptional profiles from their healthy counterparts. These findings highlight that TI-Treg cells have transcriptional profiles linked to poor CRC prognosis, with subtle location-specific variations.2026/08/20GSE275756GSM8483999GSM8484009GSM8483998GSM8484019GSM8484018GSM8484017GSM8484016GSM8484015GSM8484014GSM8484013GSM8484012GSM8484011GSM8484010GSM8483989GSM8483988GSM8484008GSM8483997GSM8484007GSM8483996GSM8484006GSM8483995GSM8484005GSM8483994GSM8483993GSM8484004GSM8484003GSM8483992GSM8484002GSM8483991GSM8483990GSM8484001GSM8484023GSM8484022GSM8484000GSM8484021GSM848402019057275756Mus musculus