GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE275nnn/GSE275918/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE275918GEOGSEfalseWhole brain transcriptome analysis of heterozygous and homozygous Chd8 mutation (Asn2373LysfsX2) mice age of P0, P25 and P56 with C57BL6/J x 129/Sv backgroundAutism spectrum disorders (ASD) are 4–5 times more common in males, possibly due to female protective effects (FPEs), which suggests females are less susceptible to ASD unless a genetic mutation is particularly strong. If this is the case, increasing the strength of a ASD-risk mutation above the female threshold may induce ASD symptoms both in females and males. To this end, we compared male and female phenotypes in heterozygous and homozygous mice carrying a patient-derived CHD8 mutation (Asn2373LysfsX2), using a hybrid genetic background to overcome homozygous lethality. Heterozygous Chd8+/N2373K males and females displayed sexually dimorphic phenotypes stronger than those in homozygous Chd8 N2373 /N2373K males and females in behaviors, brain blood flow, neuronal activity, synaptic transmission, and transcriptomes. These results suggest that a stronger Chd8 mutation suppresses sexual dimorphism in mice, supporting the FPE hypothesis in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 musculus[42106518]