GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE27nnn/GSE27596/primaryOK2000000GenomicsMus musculus Homo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27596GEOGSE0falseDifferential expression of glycosyltransferases in P19 mouse embryonal carcinoma cells with or without differentiation by retinoic acidThe project is aimed at understanding the role of the Lex-containing L5 carbohydrate epitope in the developmental process of neural induction. We plan to identify 1) the protein to which the L5 glycan is attached, 2) glycosyltransferases upregulated during neural induction, 3) the corresponding L5 carbohydrate binding protein (CBP), 4) the mechanism of interaction between L5 and its CBP, and 5) the spatiotemporal regulation of L5 and L5-CBP expression during neural induction. There is evidence, by Westernblot and immunofluorescence, that the expression of the Lewis-x-like L5 carbohydrate epitope shows dramatic differences in expression between undifferentiated cells and cells differentiated with retinoic acid. We wish to know which enzyme(s) involved in the biosynthetic pathway may be up/downregulated under these experimental conditions.2011/03/02GSE27596GSM684142GSM684131GSM684140GSM684138GSM684137GSM6841341109427596Mus musculus Homo sapiens