{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE277nnn/GSE277367/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"," Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277367"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The functional antagonism between SIRT2 and MOF regulates cell cycle progression and genome stability [ChIP-seq]","description":"The control of G2/M transition is a key event for genome stability as it ensures proper completion of DNA replication and repair before progressing into mitosis. One of the key regulators of G2/M checkpoint is the H4K16Ac-acetyltransferase MOF, which plays a major role in chromatin structure, gene expression, DNA damage and genome stability. Here we show that SIRT2, a member of the sirtuin family of NAD+-dependent deacetylases, antagonizes the role of MOF in G2/M. SIRT2 promotes specific inactivation of MOF through deacetylation and degradation of MOF, which results in re-expression of G2/M cell-cycle genes regulated by MOF. Underscoring the functional relevance of this antagonism, both factors play opposed roles in the deposition of the key mark H4K20me1 during G2/M. Consistently, loss of MOF in wt but not in SIRT2-/- cells induces a genome-wide deregulation of H4K20me1 distribution, which results in a premature loading of condensins. Our studies suggest that the G2/M checkpoint is shaped by the balance between both factors and involves condensing regulation and underscores the role of sirtuins in cell cycle control and genome stability under stress.","dates":{"publication":"2026/05/06"},"accession":"GSE277367","cross_references":{"GSM":["GSM8521150","GSM8521139","GSM8521128","GSM8521129","GSM8521148","GSM8521137","GSM8521149","GSM8521127","GSM8521138","GSM8521146","GSM8521135","GSM8521147","GSM8521136","GSM8521144","GSM8521133","GSM8521145","GSM8521134","GSM8521142","GSM8521131","GSM8521132","GSM8521143","GSM8521140","GSM8521141","GSM8521130"],"GPL":["17021","23227"],"GSE":["277367"],"taxon":["Mus musculus"," Homo sapiens"]}}