{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":[" Mus musculus","Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277368"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The functional antagonism between SIRT2 and MOF regulates cell cycle progression and genome stability [RNA-seq]","description":"The control of G2/M transition is a key event for genome stability as it ensures proper completion of DNA replication and repair before progressing into mitosis. One of the key regulators of G2/M checkpoint is the H4K16Ac-acetyltransferase MOF, which plays a major role in chromatin structure, gene expression, DNA damage and genome stability. Here we show that SIRT2, a member of the sirtuin family of NAD+-dependent deacetylases, antagonizes the role of MOF in G2/M. SIRT2 promotes specific inactivation of MOF through deacetylation and degradation of MOF, which results in re-expression of G2/M cell-cycle genes regulated by MOF. Underscoring the functional relevance of this antagonism, both factors play opposed roles in the deposition of the key mark H4K20me1 during G2/M. Consistently, loss of MOF in wt but not in SIRT2-/- cells induces a genome-wide deregulation of H4K20me1 distribution, which results in a premature loading of condensins. Our studies suggest that the G2/M checkpoint is shaped by the balance between both factors and involves condensing regulation and underscores the role of sirtuins in cell cycle control and genome stability under stress.","dates":{"publication":"2026/05/06"},"accession":"GSE277368","cross_references":{"GSM":["GSM8521160","GSM8521161","GSM8521159","GSM8521168","GSM8521157","GSM8521158","GSM8521166","GSM8521155","GSM8521156","GSM8521167","GSM8521153","GSM8521164","GSM8521154","GSM8521165","GSM8521151","GSM8521162","GSM8521163","GSM8521152"],"GPL":["17021","23227"],"GSE":["277368"],"taxon":[" Mus musculus","Homo sapiens"]}}