{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE277nnn/GSE277603/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"," synthetic construct"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277603"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Differential IRES-initiated translation during homeostatic stem cell differentiation and stress","description":"Cell stress commonly induces preferentially reduced m7G-cap-dependent translation initiation while cap-indendepent, IRES-mediated initiation persists. We investigated differential translation initiation in the highly regulated protein production of somatic stem cells by generating a reporter mouse quantifying the ratio of IRES-mediated to cap-dependent translation in vivo. Both hematopoietic and epithelial stem cells exhibited minimal IRES utilization that progressively increased with differentiation. Notably, even within defined differentiation states, lower IRES/Cap was associated with increased clonogenicity regardless of cell cycle and among HSCs, with greater long-term multi-lineage regeneration. The RNA binding protein PTBP1 is a key modulator of IRES utilization during differentiation by CRISPR/Cas9i screening. Even within IRES-low immature stem/progenitor cells, nutrient stress upregulated IRES utilization, pointing to distinct roles of IRES in cell behavior depending on physiologic state.","dates":{"publication":"2026/04/02"},"accession":"GSE277603","cross_references":{"GSM":["GSM8525881","GSM8525882","GSM8525860","GSM8525883","GSM8525861","GSM8525862","GSM8525884","GSM8525885","GSM8525863","GSM8525886","GSM8525864","GSM8525865","GSM8525887","GSM8525888","GSM8525866","GSM8525889","GSM8525867","GSM8525868","GSM8525869","GSM8525880","GSM8525870","GSM8525892","GSM8525893","GSM8525871","GSM8525872","GSM8525873","GSM8525874","GSM8525875","GSM8525876","GSM8525877","GSM8525878","GSM8525879","GSM8525858","GSM8525859","GSM8525890","GSM8525891"],"GPL":["19057","15228"],"GSE":["277603"],"taxon":["Mus musculus"," synthetic construct"]}}