GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE278nnn/GSE278556/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE278556GEOGSEfalseMechanosensing by T cells promotes a tissue-resident memory transcriptional programCell migration and strategic positioning within tissues is critical for the rapid mobilization of a T cell response. T cells must remain motile in both lymphoid and non-lymphoid tissues, which vary widely in mechanical properties such as stiffness. Here we showed that activated T cells sensed mechanical cues and responded with changes in cell morphology, nuclear envelope composition and initiation of DNA repair to protect their genomic material from force-mediated damage. Increased mechanical input also drove the transcriptional reprogramming of activated T cells, including changes in many of the core genes shared by tissue resident memory T (TRM) cells across diverse tissues, by modulating the expression of the TRM cell-associated transcription factors Klf2, Runx3 and Hic1. Thus, mechanosensing by activated T cells impacted T cell fate, promoting a transcriptional program associated with tissue-residency.2026/06/02GSE278556GSM8548807GSM8548806GSM8548809GSM8548808GSM8548814GSM8548813GSM8548805GSM8548816GSM8548815GSM8548810GSM8548812GSM854881124247278556Mus musculus