{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE279nnn/GSE279020/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE279020"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"m6A-eCLIP sequencing in mouse alpha cell line aTC1-6 cells with Scramble or Mettl14 KD","description":"N6-methyladenosine (m6A) is a widespread RNA modification that plays a crucial role in regulating gene expression, affecting processes such as RNA stability, splicing, and translation. I Previous studies have shown that m6A is involved in fine-tuning β-cell function, but its impact on α-cells, which secrete glucagon in response to low glucose and amino acids, has not been fully investigated. Here, we show that METTL14, a key m6A writer, is essential for maintaining α-cell function and identity. Using a combination of in vitro and in vivo models, we demonstrate that metabolic stimuli such as low glucose and amino acids upregulate METTL14, enhancing glucagon secretion, while insulin downregulates it. Importantly, α-cell-specific knockout of Mettl14 in mice results in impaired glucagon secretion, increased insulin levels, and a surprising conversion of α-cells into β-cells. These findings reveal that m6A is a critical regulator of α-cell identity and function, expanding our understanding of RNA modifications in islet biology. By uncovering a role for METTL14 in maintaining α-cell integrity, our study opens new avenues for exploring RNA-based therapies in diabetes and other metabolic disorders.","dates":{"publication":"2026/07/08"},"accession":"GSE279020","cross_references":{"GSM":["GSM8559707","GSM8559708","GSM8559703","GSM8559704","GSM8559705","GSM8559706","GSM8559701","GSM8559702"],"GPL":["30172"],"GSE":["279020"],"taxon":["Mus musculus"]}}