{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE279nnn/GSE279734/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE279734"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Notch withdrawal unlocks emergence of CD4 T cells from human iPSCs","description":"In the human thymus, abT cell progenitors differentiate into both CD4+ helper cells and CD8+ cytotoxic cells. T cell progenitors derived from induced pluripotent stem cells fail to effectively differentiate into CD4+ helper cells. Here we describe a key role for the withdrawal of Notch ligand during the final step of stimulation through the T cell receptor to prompt T cell maturation allowing access to the CD4 lineage in iPSC T cells (iT cells). These CD4+ iT cells produce signature CD4 cytokines and express a transcriptional signature similar to human blood CD4 T cells. We believe this is a key step towards iT cell therapies incorporating both helper and cytotoxic iT cells.","dates":{"publication":"2026/09/16"},"accession":"GSE279734","cross_references":{"GSM":["GSM8579404","GSM8579402","GSM8579403"],"GPL":["11154"],"GSE":["279734"],"taxon":["Homo sapiens"],"PMID":["[42660120]"]}}