GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE279nnn/GSE279734/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE279734GEOGSEfalseNotch withdrawal unlocks emergence of CD4 T cells from human iPSCsIn the human thymus, abT cell progenitors differentiate into both CD4+ helper cells and CD8+ cytotoxic cells. T cell progenitors derived from induced pluripotent stem cells fail to effectively differentiate into CD4+ helper cells. Here we describe a key role for the withdrawal of Notch ligand during the final step of stimulation through the T cell receptor to prompt T cell maturation allowing access to the CD4 lineage in iPSC T cells (iT cells). These CD4+ iT cells produce signature CD4 cytokines and express a transcriptional signature similar to human blood CD4 T cells. We believe this is a key step towards iT cell therapies incorporating both helper and cytotoxic iT cells.2026/09/16GSE279734GSM8579404GSM8579402GSM857940311154279734Homo sapiens[42660120]