GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE280nnn/GSE280469/primaryOK200OtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE280469GEOGSEfalseP5CS regulates translation by functioning as non-canonical RBP [Ribo-seq]P5CS is the rate-limiting enzyme in the proline biosynthetic pathway, while whether P5CS mediates function beyond enzyme activity remains completely unknown. Here, we reveal that P5CS functions as a non-canonical RNA-binding protein and inhibits the growth and metastasis of cancer cells by suppressing translation initiation in an enzyme activity-independent manner. To explore the underlying mechanism of P5CS in translation regulation, Ribo-seq, RIP-seq and LACE-seq were performed to investigate the downstream targets of P5CS.2026/06/09GSE280469GSM8598397GSM8598398GSM8598393GSM8598394GSM8598395GSM859839624676280469Homo sapiens