{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE280nnn/GSE280531/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE280531"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Shaping Gut Immunity with Intestinal Epithelial-Immune Dynamics in Peyer's Patches.","description":"Microfold (M) cells are specialized antigen-sampling cells that transcytose luminal antigens to underlying immune cells to initiate mucosal immunity. How they are programmed in different intestinal tissues and their role during enteric infections has not been resolved. Here, we demonstrate that M cells are critical for B cell activation and IgA production that shapes the intestinal microbiome and maintains steady-state intestinal epithelial integrity. Tuft cells and M cells share expression of SPI-B. Molecular mapping revealed that M cells exhibit significant heterogeneity including tissue-specific and pathogen-specific tailored programs. These include key pathways regulating fatty acid uptake and metabolism in the intestinal epithelium. Confocal imaging demonstrated that Peyer’s patch SPI-B+ M cells act to retain group 3 innate lymphoid cells and IL-22 production within the Peyer's patch epithelium. By identifying interconnected cellular and molecular programs, we uncover novel spatially organized immune-epithelial regulatory networks essential for immune protection.","dates":{"publication":"2026/06/29"},"accession":"GSE280531","cross_references":{"GSM":["GSM8599882","GSM8599881"],"GPL":["30172"],"GSE":["280531"],"taxon":["Mus musculus"]}}