{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE280nnn/GSE280744/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE280744"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Peyer’s patch M cells organize an epithelial niche that sustains group 3 innate lymphoid cells and IL-22","description":"Microfold (M) cells transcytose luminal antigens to initiate mucosal adaptive immunity, but their role in organizing innate responses in Peyer's patches is unclear. Here we showed that Peyer's patch M cells organized an epithelial-group 3 innate lymphoid cell (ILC3) axis, establishing a spatial niche within the dome epithelium that drove ILC3 localization, proliferation and IL-22 production. We found that epithelial, but not hematopoietic, SPI-B was required for intestinal IgA responses to establish this niche. Single-cell profiling of intestinal SPI-B+ epithelial cells revealed that M cells were highly heterogeneous, displaying tissue- and pathogen-specific transcriptional programs. Using subset-specific genetic perturbation and whole-mount imaging, we found that this circuit relied on CCR6-dependent positioning cues and RANK-RANKL signaling to ILC3 to regulate Peyer's patch ILC3 homeostasis. Together, these findings identified Peyer's patch M cells as organizers that spatially coordinated innate cell localization, proliferation and cytokine production to maintain mucosal barrier defense.","dates":{"publication":"2026/06/26"},"accession":"GSE280744","cross_references":{"GSM":["GSM8599882","GSM8599881","GSM7790630","GSM7790634","GSM7790633","GSM7790632","GSM7790631","GSM7790627","GSM7790626","GSM7790636","GSM7790625","GSM7790635","GSM7790629","GSM7790628"],"GPL":["19057","30172"],"GSE":["280744"],"taxon":["Mus musculus"],"PMID":["[42601474]"]}}