{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE281nnn/GSE281223/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Methylation profiling"],"species":["Mus musculus"],"gds_type":["Methylation profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE281223"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Chemical tracking of DNMT1-specific activity during mESC ground state transition using WGBS","description":"The enzymatic methylation of cytosine to 5-methylcytosine in DNA is a crucial epigenetic process implicated in mammalian development and disease. DNA methylation results from the collective performance of three AdoMet-dependent DNA methyltransferases (DNMTs), whose catalytic interplay and temporal dynamics remain poorly elucidated. In this study, we generated a Dnmt1 knock-in mESC line with a N1580A mutation at a single allele, while retaining a functioning wild-type protein. The heterozygous DNMT1 N1580A knock-in line, together with the DNMT1 wild-type, homozygous N1580A knock-in, and knockout mouse embryonic stem cells were subsequently assessed for genome-wide CpG methylation levels using whole-genome bisulfite sequencing. Next, monoallelic DNMT1 N1580A KI cells underwent electroporation using the synthetic cofactor analogue Ado-6-azide and genomic targets unique to DNMT1 were subsequently assessed with Dnmt-TOP-seq. Finally, a comprehensive examination of DNMT1-specific events during mESC ground state transition was conducted. Taken together, the present study paves the way for in vivo monitoring of DNMT1 activity in living tissues in a variety of biological contexts.","dates":{"publication":"2026/08/01"},"accession":"GSE281223","cross_references":{"GSM":["GSM8615135","GSM8615136","GSM8615133","GSM8615134"],"GPL":["24247"],"GSE":["281223"],"taxon":["Mus musculus"]}}