{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE281nnn/GSE281387/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE281387"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effects of OGT-ISS deletion on gene expression in NK92 cells","description":"O-GlcNAcylation, a type of post-translational modification, is known to regulate the functions of various immune cells. In this study, we report that increasing O-GlcNAcylation enhances the tumor-killing efficacy of NK cells. By removing the ISS (intronic splicing silencer) of OGT in NK92 cells, we achieved stabilized O-GlcNAcylation even under tumor microenvironment (TME)-mimicking conditions, which led to a heightened killing effect against tumors. To investigate how OGT-ISS deletion impacts gene expression profiles associated with NK cell activity, we performed RNA sequencing (RNA-seq) to compare the global transcriptomes of wild-type (WT) and ΔOGT-ISS_NK92 cells. This comparative transcriptome analysis aims to identify gene expression profiles that influence NK cell cytotoxicity and functional responses in the context of tumor immunity.","dates":{"publication":"2026/06/10"},"accession":"GSE281387","cross_references":{"GSM":["GSM8619753","GSM8619754","GSM8619755","GSM8619756"],"GPL":["24676"],"GSE":["281387"],"taxon":["Homo sapiens"],"PMID":["[40914422]"]}}