{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE282nnn/GSE282031/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Genome binding/occupancy profiling by high throughput sequencing"," Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE282031"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"BRD4 O-GlcNAcylation attenuates phase separation to regulate transcription","description":"BRD4 undergoes liquid-liquid phase separation (LLPS) at super-enhancers (SEs) to facilitate gene control. However, the regulatory mechanisms and transcriptional consequences of BRD4 condensation remain elusive. Here, we show that the BRD4 C-terminal intrinsically disordered region (IDR) is a primary interacting region for O-GlcNAc transferase (OGT), enabling O-GlcNAc modifications at 12 potential sites. O-GlcNAcylation attenuates BRD4 LLPS, leading to small puncta and increased fluidity, which significantly declines occupancy and transcription at SEs. We further demonstrate that loss of O-GlcNAcylation on BRD4 led to elevated gene expression relating to cell cycle and DNA damage, allowing cells less sensitive to DNA damage and promoting faster growth. Therefore, we identify an O-GlcNAc–mediated regulatory mechanism for BRD4 LLPS that governs gene control at SEs, showcasing how O-GlcNAc modifications can influence transcriptional activities at SEs through a phase separation model.","dates":{"publication":"2026/07/29"},"accession":"GSE282031","cross_references":{"GSM":["GSM8634662","GSM9787157","GSM9787156","GSM8634660","GSM8634661","GSM8634644","GSM9787153","GSM9787152","GSM8634645","GSM9787155","GSM8634642","GSM8634643","GSM9787154","GSM8634648","GSM8634649","GSM8634646","GSM8634647","GSM9038251","GSM9038250","GSM9038253","GSM9038252","GSM9787151","GSM9787150","GSM8634651","GSM8634652","GSM8634650","GSM8634655","GSM9038248","GSM9038247","GSM8634656","GSM8634653","GSM9038249","GSM8634654","GSM8634659","GSM8634657","GSM9038246","GSM8634658","GSM9787149"],"GPL":["24676","29480"],"GSE":["282031"],"taxon":["Homo sapiens"]}}