{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE283nnn/GSE283389/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"," Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE283389"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput functional characterization of enhancers in totipotent-like cells","description":"Zygotic genome activation (ZGA) marks the initial transcription event in embryogenesis, yet the cis-regulatory mechanism remains unclear. Here, utilizing massively parallel reporter assays, we functionally dissect enhancers across mouse genome in DUX-induced 2C-like cells (2CLCs). Through integrated analysis with epigenomic and transcriptomic data from 2CLCs and 2C embryos, active enhancers in totipotent cells are depicted. Among them, a notable proportion of promoters exhibit enhancer activities, showing elevated active chromatin features and correlating with enhanced gene expression during ZGA. Transcription factors preferentially enriched at enhancer-promoter regions play critical roles in regulating ZGA and early embryonic development. Furthermore, only half of the MT2_Mm exhibit enhancer activities in 2CLCs. Notably, 2CLC enhancers augment transcription in 2C embryos. Finally, deleting enhancer regions in both 2CLCs and 2C embryos, together with dCas9-KRAB-MeCP2-mediated CRISPRi in vitro, collectively underscores their crucial role in facilitating transcription of ZGA genes. These findings advance our comprehension of the cis-regulatory mechanism governing ZGA process.","dates":{"publication":"2026/07/19"},"accession":"GSE283389","cross_references":{"GSM":["GSM8660598","GSM8660597","GSM8660596","GSM8660595","GSM8660613","GSM8660612","GSM8660611","GSM8660610","GSM8660599","GSM8660594","GSM8660593","GSM8660592","GSM9797981","GSM9797980","GSM8660606","GSM8660605","GSM8660604","GSM8660626","GSM8660603","GSM8660625","GSM8660609","GSM8660608","GSM8660607","GSM8660620","GSM8660624","GSM8660602","GSM8660601","GSM8660623","GSM8660600","GSM8660622","GSM8660621","GSM8660617","GSM8660616","GSM8660615","GSM8660614","GSM8660619","GSM8660618"],"GPL":["17021","21273","24247"],"GSE":["283389"],"taxon":["Mus musculus"]}}