{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE284nnn/GSE284015/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE284015"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"GZMK-expressing tissue-resident memory CD8+ T cells mediate human intestinal acute graft-versus-host disease","description":"Tissue-resident memory T cells (TRM) have recently emerged as crucial cellular players for murine acute graft-versus-host disease (aGVHD) development. However, the role of distinct subsets of CD8+TRM cells in human intestinal aGVHD is not well known yet. Herein,we combined multiplex immunohistochemical staining with single-cell RNA sequencing to elucidate the differentiation trajectory, lineage commitment, clonal expansion and functional properties of distinct CD8+TRM cell subsets in human intestinal aGVHD. We revealed seven functionally distinct CD8+TRM subsets and innovatively uncovered a significant GZMK+CD8+TRM subset. GZMK+CD8+TRM subset mediated human intestinal aGVHD by exhibiting enhanced clonal expansion, IFN signaling pathway-associated proinflammatory, and lineage bifurcation differentiation properties. High GZMK+CD8+TRM subset infiltration is correlated with human intestinal aGVHD severity and poor prognosis. Therefore, we demonstrated that human intestinal aGVHD is in good part mediated by pivotal GZMK+CD8+TRM subset. Targeting key CD8+TRM cell subsets could be a new treatment approach in the human intestinal aGVHD.","dates":{"publication":"2026/05/06"},"accession":"GSE284015","cross_references":{"GSM":["GSM8676180","GSM9060920","GSM8676184","GSM8676183","GSM8676182","GSM8676181","GSM8676188","GSM8676187","GSM8676186","GSM8676185","GSM8676189","GSM8676191","GSM8676190","GSM8676195","GSM8676194","GSM8676193","GSM8676192","GSM9060919","GSM8676177","GSM8676176","GSM8676197","GSM8676175","GSM8676174","GSM8676196","GSM8676179","GSM8676178"],"GPL":["24676"],"GSE":["284015"],"taxon":["Homo sapiens"]}}