GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE284nnn/GSE284364/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE284364GEOGSEfalseRest-phase light disrupts hippocampal clock function and impairs object recognition memoryLight exposure during the circadian rest phase is common during shift work and late-night activity, but its effects on memory remain unclear. Here, we show that 6-day rest-phase light exposure (RPL), which combines mistimed light exposure with enforced wakefulness (EW) during the circadian rest phase, impairs long-term object recognition memory but not short-delay spatial memory. Behavioral testing, single-nucleus RNA sequencing, and ex vivo electrophysiology show that RPL alters SCN clock organization and output signaling, reduces hippocampal clock-gene rhythmicity, disrupts phase-dependent synaptic gene expression in dentate gyrus and CA1 neurons, and reduces CA1 long-term potentiation. Maintaining EW without light exposure preserved object recognition memory and CA1 clock-gene expression, showing that mistimed light exposure is required for both effects in this model. Local hippocampal dexamethasone treatment also restored day–night differences in hippocampal clock-gene expression and preserved memory under RPL, supporting a role for hippocampal clock-related regulation in memory preservation. Together, these findings show that RPL alters clock-related regulation in the SCN and hippocampus and impairs object recognition memory.2026/08/14GSE284364GSM8682860GSM8682861GSM8682854GSM8682855GSM8682856GSM8682857GSM8682858GSM868285921273284364Mus musculus