{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE284nnn/GSE284422/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE284422"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Genuine-seq enables genome-wide unbiased identification of off-target cleavage and translocation by CRISPR nucleases, II","description":"CRISPR-Cas system is one of the most widely used tool kit for genome editing. In practice, CRISPR’s causes chromosomal structural variation due to off-targets, which is a serious genotoxicity, while currently unbiased methods for detecting chromosomal structural variation are largely lacking. Here, we developed Genome-wide ubiased identification of nuclease effects by sequencing (Genuine-Seq), which could detect both off-target sites of CRISPR and chromosomal structural variations caused by off-target effects, including translocations and large deletions. We demonstrated Genuine-seq’s high efficiency in human cells, and compared spCas9 and spRY, emphasizing that spRY can cause tremendous off-target cutting. Using mouse models, we performed in vivo experiments to depict the landscape of off-target sites and chromosomal structural variations caused by CRISPR-Cas editing, which demonstrated the translational value of Genuine-seq in safety assessment of CRISPR-based therapeutics.","dates":{"publication":"2026/09/26"},"accession":"GSE284422","cross_references":{"GSM":["GSM8683895","GSM8683896","GSM8683897","GSM8683898","GSM8683899","GSM8683900","GSM8683901","GSM8683902"],"GPL":["34284"],"GSE":["284422"],"taxon":["Homo sapiens"]}}