GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285235/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE285235GEOGSEfalseR-loop maintains the deposition of H3K36me3 to prevent invasion of H3K4me3 during SN-oocyte development [RNA-Seq]Establishment of epigenetic modifications in an orderly manner is crucial for oocyte maturation and embryonic development. R-loop (DNA/RNA hybrids) plays an important role in regulating genome stability, epigenetic modifications and gene expression. However, the specific functions and regulatory mechanisms of R-loop on the crosstalk between histone modifications during oocyte development remain unclear. Here, we find that loss of R-loop by overexpressing Rnaseh1 significantly slow down oocyte development. R-loop loss in germinal vesicle (GV) oocyte leads to downregulation of H3K36me3 at key oocyte developmental gene bodies, aberrant invasion of H3K4me3 into H3K36me3-marked regions, resulting in dysregulated gene expression and ultimately hindering oocyte development. Importantly, this dysregulated gene expression can be rescued by histone H3K36me3 methyltransferase SETD2 targeting on specific R-loop-dependent H3K36me3-marked regions. Together, this finding reveals that R-loop acts as a crucial regulator in controlling epigenetic crosstalk of H3K36me3 and H3K4me3 in maternal genome, ensuring normal oocyte development.2026/07/13GSE285235GSM8747216GSM8747217GSM8747214GSM8747215GSM8747212GSM8747213GSM8698939GSM8698938GSM8698942GSM8698941GSM8698944GSM8698943GSM8698945GSM869894028330285235Mus musculus