{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285236/suppl/GSE285236_mCherry_CpG.bw","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285236/suppl/GSE285236_Rnaseh1_CpG.bw","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285236/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE285236"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"R-loop maintains the deposition of H3K36me3 to prevent invasion of H3K4me3 during SN-oocyte development [EM-Seq]","description":"Establishment of epigenetic modifications in an orderly manner is crucial for oocyte maturation and embryonic development. R-loop (DNA/RNA hybrids) plays an important role in regulating genome stability, epigenetic modifications and gene expression. However, the specific functions and regulatory mechanisms of R-loop on the crosstalk between histone modifications during oocyte development remain unclear. Here, we find that loss of R-loop by overexpressing Rnaseh1 significantly slow down oocyte development. R-loop loss in germinal vesicle (GV) oocyte leads to downregulation of H3K36me3 at key oocyte developmental gene bodies, aberrant invasion of H3K4me3 into H3K36me3-marked regions, resulting in dysregulated gene expression and ultimately hindering oocyte development. Importantly, this dysregulated gene expression can be rescued by histone H3K36me3 methyltransferase SETD2 targeting on specific R-loop-dependent H3K36me3-marked regions. Together, this finding reveals that R-loop acts as a crucial regulator in controlling epigenetic crosstalk of H3K36me3 and H3K4me3 in maternal genome, ensuring normal oocyte development.","dates":{"publication":"2026/07/13"},"accession":"GSE285236","cross_references":{"GSM":["GSM8698946","GSM8698948","GSM8698947","GSM8698949"],"GPL":["28330"],"GSE":["285236"],"taxon":["Mus musculus"]}}