GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE286nnn/GSE286905/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE286905GEOGSEfalseSB431542 conditioned hASCs-derived extracellular vesicles enhance in vitro and in vivo osteogenesis via a miR20-a-5p-BAMBI-BMP/Wnt signaling axisCritical-sized bone defects resulting from tumors, trauma, or surgery present a significant clinical challenge due to its poor self-regenerating capabilities and limited therapeutic options. Mesenchymal stem cells (MSCs) have shown promise in bone regeneration, possibly mediated through their secretion of bioactive factors rather than direct engraftment and differentiation. MSC-derived extracellular vesicles (EVs) deliver micro-RNAs (miRs), proteins and lipids that may modulate bone regeneration. EVs were isolated by differential ultracentrifugation from osteogenically induced human adipose tissue-derived MSCs (hASCs), treated with or without the small molecule SB431542, an inhibitor of TGFß-signaling (EV-hASCs[-/+]SB). EVs were characterized using Western Blot and Nanoparticle Tracking Analysis. hASCs were cultured with EV-hASCs[-/+]SBs to assess bone regeneration in vitro. Bone regeneration was assessed in vivo in calvarial defects treated with (EV-hASCs[-/+]SB). EV content was analyzed via miR-analysis and gain- and loss-of-function transfection assays were performed with identified miR. Enhanced osteogenesis is shown in vitro and in vivo upon treatment with EV-hASCs[+]SB. EV-hASCs[-/+]SB-enriched miR-20a-5p was identified as a modulating factor in osteogenesis, silencing BAMBI and enhancing early downstream phosphorylation of SMAD1/5 and late activation of ß-Catenin in ASCs undergoing osteogenic differentiation, targets of BMP- and Wnt-signaling pathways. EVs act as carriers for miR-mediated paracrine signaling, harnessing the therapeutic potential of MSCs, while circumventing the high immunogenicity and risk of neoplastic transformation associated with cell-based therapies.2026/04/30GSE286905GSM8733589GSM8733588GSM8733587GSM873359018573286905Homo sapiens