{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE288nnn/GSE288017/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE288017"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Mouse embryoid body differentiation in Profilin-2 3'UTR cis-element mutants","description":"The coordinated actions of post-transcriptional regulation are essential for stem cell differentiation. The Profilin-2 transcript can be targeted by microRNAs and RNA binding proteins, specifically Ago2 and ESCC family of miRNAs -- miR-291-3p/294-3p/295-3p/302-3p -- and the Iron Regulatory Proteins IRP1/2. Earlier studies described the role of the conserved Profilin-2 ESCC miRNA site. This study describes the role of the conserved Profilin-2 IRP/iron-response-element site by CRISPR-mediated disruption of the Profilin-2 3'UTR iron response element site. The transcriptomic data reported indicates impaired germ layer formation and distribution during embryoid body differentiation when compared to wild-type mouse embryonic stem cells.","dates":{"publication":"2026/08/18"},"accession":"GSE288017","cross_references":{"GSM":["GSM8457698","GSM8457699","GSM8457697","GSM8457708","GSM8457706","GSM8457707","GSM8457704","GSM8457705","GSM8457702","GSM8457703","GSM8457700","GSM8457701"],"GPL":["21103"],"GSE":["288017"],"taxon":["Mus musculus"]}}