GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE288nnn/GSE288017/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE288017GEOGSEfalseMouse embryoid body differentiation in Profilin-2 3'UTR cis-element mutantsThe coordinated actions of post-transcriptional regulation are essential for stem cell differentiation. The Profilin-2 transcript can be targeted by microRNAs and RNA binding proteins, specifically Ago2 and ESCC family of miRNAs -- miR-291-3p/294-3p/295-3p/302-3p -- and the Iron Regulatory Proteins IRP1/2. Earlier studies described the role of the conserved Profilin-2 ESCC miRNA site. This study describes the role of the conserved Profilin-2 IRP/iron-response-element site by CRISPR-mediated disruption of the Profilin-2 3'UTR iron response element site. The transcriptomic data reported indicates impaired germ layer formation and distribution during embryoid body differentiation when compared to wild-type mouse embryonic stem cells.2026/08/18GSE288017GSM8457698GSM8457699GSM8457697GSM8457708GSM8457706GSM8457707GSM8457704GSM8457705GSM8457702GSM8457703GSM8457700GSM845770121103288017Mus musculus