GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE288nnn/GSE288621/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE288621GEOGSEfalseComparative Single Cell RNA Sequencing of Notch2 and RBPJ-deficient mononuclear phagocyte cells from the spleenSplenic mononuclear phagocytes are critical in various homeostatic processes as well as immune responses. We employed single-cell RNA sequencing to investigate mononuclear phagocytic cell subsets, including splenic red pulp macrophages, monocytes, and dendritic cells in the spleen, with a focus on how Notch2 and its nuclear mediator Rbpj regulate these subsets. Our study offers a comparative analysis of splenic mononuclear phagocytes and specifically red pulp macrophages examining phagocytes isolated from myeloid Notch2 (N2ΔCx3cr1)-, and Rbpj (RbpjΔCx3cr1)-deficient mice or control animals. Our findings may provide further insights into the heterogeneity and development of the splenic mononuclear phagocytic system including splenic macrophages and monocytes.2026/08/18GSE288621GSM8771408GSM877140719057288621Mus musculus