GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE289nnn/GSE289932/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE289932GEOGSEfalseAnti-inflammatory and phosphorylation effects of CFTR modulator triple therapy in cystic fibrosisCystic Fibrosis is an autosomal recessive disorder caused by mutations in the CFTR gene, which encodes a key chloride channel, leading to impaired ion transport and severe progressive respiratory failure. CFTR modulators, such as Trikafta© has improved morbidity in CF, but its systemic effects remain unclear. This study evaluated the clinical outcomes, safety, and cellular mechanisms of Trikafta© in young children (< 11 years) with CF using a multi-omic approach. Seventeen children with CF (median age 8.7 years, 26% female) and 12 healthy controls (median age 9.6 years, 42% female) were included for multi-omic analysis. The analysis included RNA sequencing, proteomics and phosphoproteomics. Baseline pathway enrichment analysis (FDR < 0.05) revealed systemic inflammation, transmembrane transporter deficiencies, reduced protein kinase, and reduced GTPase activity. Three months post-Trikafta© treatment, anti-inflammatory effects, epigenetic modulation, and increased protein kinase activity were observed, indicating partial reversal of cellular abnormalities. Trikafta© improves clinical outcomes and reduces inflammation, epigenetic changes and key phosphorylation pathways in young children with CF, offering insights into CF pathology and potential biomarkers for monitoring treatment.2026/08/03GSE289932GSM8801794GSM8801795GSM8801796GSM8801830GSM8801797GSM8801831GSM8801798GSM8801810GSM8801799GSM8801832GSM8801811GSM8801833GSM8801834GSM8801812GSM8801813GSM8801814GSM8801815GSM8801816GSM8801817GSM8801818GSM8801819GSM8801791GSM8801792GSM8801793GSM8801820GSM8801821GSM8801822GSM8801800GSM8801823GSM8801801GSM8801802GSM8801824GSM8801825GSM8801803GSM8801826GSM8801804GSM8801805GSM8801827GSM8801828GSM8801806GSM8801807GSM8801829GSM8801808GSM880180924676289932Homo sapiens[42707060]