{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE290nnn/GSE290248/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE290248"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection","description":"Alternative polyadenylation (APA) is a widespread mechanism of gene regulation that generates transcript isoforms with distinct 3' ends. While several RNA-binding proteins (RBPs) are known to regulate APA, the roles of the vast majority of RBPs in this process remain poorly characterized. Using a large scale tethered function assay, we screened a library of 879 human RBPs to identify regulators of APA. We discovered 52 RBPs that modulate APA, validated their functions through knockdown PAS-seq and enhanced crosslinking and immunoprecipitation (eCLIP), and developed a fine-tuned transformer model to predict activators of PAS selection. We focused on two unexpected hits: GRB2, an adaptor protein with crucial roles in cell signaling, and RNPS1, a member of the exon junction complex (EJC). We uncovered mechanisms by which they modulate APA using affinity purification mass spectrometry (AP-MS) and biochemical assays. Our screennprovides a comprehensive resource of RBPs involved in APA and reveals unexpected roles of signaling factors in APA regulation.","dates":{"publication":"2026/06/29"},"accession":"GSE290248","cross_references":{"GSM":["GSM8809270","GSM8809271","GSM8809272","GSM8809262","GSM8809263","GSM8809264","GSM8809265","GSM8809260","GSM8809261","GSM8809266","GSM8809267","GSM8809268","GSM8809269","GSM8809251","GSM8809252","GSM8809253","GSM8809254","GSM8809250","GSM8809259","GSM8809255","GSM8809256","GSM8809257","GSM8809258","GSM8809240","GSM8809241","GSM8809242","GSM8809243","GSM8809248","GSM8809249","GSM8809244","GSM8809245","GSM8809246","GSM8809247","GSM8809196","GSM8809230","GSM8809197","GSM8809231","GSM8809198","GSM8809199","GSM8809232","GSM8809192","GSM8809193","GSM8809194","GSM8809195","GSM8809237","GSM8809238","GSM8809239","GSM8809233","GSM8809234","GSM8809235","GSM8809236","GSM8809190","GSM8809191","GSM8809185","GSM8809186","GSM8809187","GSM8809220","GSM8809188","GSM8809221","GSM8809181","GSM8809182","GSM8809183","GSM8809184","GSM8809226","GSM8809227","GSM8809228","GSM8809229","GSM8809222","GSM8809189","GSM8809223","GSM8809224","GSM8809225","GSM8809219","GSM8809180","GSM8809176","GSM8809210","GSM8809177","GSM8809215","GSM8809216","GSM8809217","GSM8809218","GSM8809211","GSM8809178","GSM8809179","GSM8809212","GSM8809213","GSM8809214","GSM8809208","GSM8809209","GSM8809204","GSM8809205","GSM8809206","GSM8809207","GSM8809200","GSM8809201","GSM8809202","GSM8809203"],"GPL":["24676"],"GSE":["290248"],"taxon":["Homo sapiens"],"PMID":["[42361791]"]}}