{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE290nnn/GSE290281/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE290281"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection [eCLIP]","description":"Alternative polyadenylation (APA) is a widespread mechanism of gene regulation that generates transcript isoforms with distinct 3' ends. While several RNA-binding proteins (RBPs) are known to regulate APA, the roles of the vast majority of RBPs in this process remain poorly characterized. Using a large-scale tethered function assay, we screened a library of 879 human RBPs to identify regulators of APA. We discovered 52 RBPs that modulate APA, validated their functions through knockdown PAS-seq and enhanced crosslinking and immunoprecipitation (eCLIP), and developed a fine-tuned transformer model to predict activators of PAS selection. We focused on two unexpected hits: GRB2, an adaptor protein with crucial roles in cell signaling, and RNPS1, a member of the exon junction complex (EJC). We uncovered mechanisms by which they modulate APA using affinity purification mass spectrometry (AP-MS) and biochemical assays. Our screen provides a comprehensive resource of RBPs involved in APA and reveals unexpected roles of signaling factors in APA regulation.","dates":{"publication":"2026/06/29"},"accession":"GSE290281","cross_references":{"GSM":["GSM8809691","GSM8809692","GSM8809693","GSM8809694","GSM8809690","GSM8809699","GSM8809710","GSM8809711","GSM8809678","GSM8809679","GSM8809712","GSM8809713","GSM8809695","GSM8809696","GSM8809697","GSM8809698","GSM8809707","GSM8809708","GSM8809709","GSM8809703","GSM8809704","GSM8809705","GSM8809706","GSM8809680","GSM8809681","GSM8809682","GSM8809683","GSM8809688","GSM8809700","GSM8809689","GSM8809701","GSM8809702","GSM8809684","GSM8809685","GSM8809686","GSM8809687"],"GPL":["24676"],"GSE":["290281"],"taxon":["Homo sapiens"],"PMID":["[42361791]"]}}