GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE290nnn/GSE290288/primaryOK200TranscriptomicsEquus asinusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE290288GEOGSEfalseDeciphering the single-cell atlas of fetal donkey (Equus asinus) gonad development in early pregnancy(1) Introduction: The ovaries are the reproductive organs of female animals and play a key role in fertility and maintaining endocrine balance. Gonadal development is a complex process that involves sex determination and subsequent maturation into the ovaries or testes. At present, the understanding of the mechanisms of donkey gonad development is still limited. (2) Objectives: This study explores the comprehensive single-cell transcriptomics of donkey gonads, providing insights into the cellular and molecular events behind sex norms and gonad differentiation. (3) Methods: We used single-cell sequencing technology to construct a high-resolution map of fetal donkey gonad development, analyze the diversity and complexity of the cell composition of donkey gonads at different developmental stages, and reveal the characteristics of key cell types and gene expression patterns. (4) Results: The different cell states and active molecular pathways during the development of donkey gonadal tissue were revealed, and the gonadal ridge developed into ovary at 4-8 weeks. (5) Conclusions: This study provides a foundational resource for understanding the complex biology of donkey gonadal development and may have implications for donkey reproductive health and fertility.2026/07/01GSE290288GSM8809853GSM880985435123290288Equus asinus