GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE291nnn/GSE291703/primary200OKTranscriptomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE291703GEOGSEfalseExpression data from 16HBE14o- cellsE-liquid flavorings are one of the driving attractors for young electronic cigarette users. The added flavors are considered safe for oral ingestion, however long-term respiratory effects remain unknown and unassessed. This study aimed to systematically investigate the intrinsic toxicity of individual flavors in a submerged lung cell model and in aerosolized form having a more complex composition using an air-liquid interface (ALI) lung cell model. 16HBE14o- bronchial epithelial cells were exposed in submerged condition for 24 h to non-cytotoxic doses of cinnamaldehyde, eugenol, vanillin and diacetyl. Cells were exposed at ALI to aerosols of self-made e-liquids containing 70 µg mL-1 cinnamaldehyde, eugenol, or vanillin for 10 min with 24 h post-exposure. Transcriptomic changes were analyzed using the Clariom™ S assay in order to understand mechanistic toxicity due to flavoring exposure.2026/09/30GSE291703GSM8840380GSM8840381GSM8840401GSM8840402GSM8840403GSM8840404GSM8840405GSM8840406GSM8840407GSM8840408GSM8840382GSM8840383GSM8840384GSM8840385GSM8840386GSM8840387GSM8840388GSM8840400GSM8840389GSM8840390GSM8840391GSM8840392GSM8840409GSM8840379GSM8840412GSM8840393GSM8840394GSM8840395GSM8840396GSM8840397GSM8840398GSM8840410GSM8840399GSM8840377GSM8840411GSM884037823159291703Homo sapiens