GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE292nnn/GSE292606/suppl/GSE292606_Metadata.xlsxftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE292nnn/GSE292606/suppl/filelist.txtftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE292nnn/GSE292606/suppl/GSE292606_RAW.tarftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE292nnn/GSE292606/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE292606GEOGSEfalseClonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [scRNAseq]Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor We characterized mPB CD34+ cell population dynamics (n=2 donors) by single cell RNA sequencing (scRNAseq) during 8 days of expansion culture using commercial serum-free media (Miltenyi Bioscience) in the presence of the epigenetic modifier UM171 and early acting cytokines (SFT6: stem cell factor, 100ng/mL; flt3 ligand, 100ng/mL; thrombopoietin, 50ng/mL; interleukin 6, 50ng/mL). Cultured cells were transduced with a lentiviral vector expressing a marker gene, to simulate a gene therapy context. CD34+ cells from donor 1 were analyzed before culture, on day 4 and on day 8 of expansion. For donor 2, in addition to the CD34+ bulk expansion culture, part of the CD34+ cells were FACS-sorted into subpopulations enriched in primitive HSPC (CD34+CD38-), committed granulocyte-monocyte progenitors (GMP) or megakaryocyte-erythrocyte progenitors (MEP), and subsequently expanded for 7 days.2026/06/10GSE292606GSM8862367GSM8862366GSM8862372GSM8862371GSM8862370GSM8862369GSM886236824676292606Homo sapiens