{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293047/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293047"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"20S proteasomes are required for ELVAs-mediated protein degradation during oocyte-to-embryo transition and aging","description":"The degradation of maternal factors is essential for oocyte-to-embryo transition (OET). While extensive research has focused on maternal mRNA degradation, the mechanisms underlying maternal protein degradation remain poorly understood. ELVAs, the oocyte-specific membraneless structure which comprises proteasomes, endolysosomes and autophagosomes, are required for protein degradation in maturing oocytes and zygotes. In this study, we embloyed oocyte-specific Psma7 knockout, targeting the α4 subunit of 20S proteasome, to investigate the physiological function of proteasomes in ELVAs-mediated protein degradation. PSMA7-deficiency destabilizes 20S proteasome components and disrupts ELVAs assembly and spatial organization, resulting in the accumulation of ubiquitinated proteins in both oocytes and zygotes. Consequently, maternal PSMA7-deletion results in female infertility, manifested by impaired oocyte maturation and developmental arrest at the 1- to 2-cell stage. Furthermore, in oocytes from both aged mice and women, reduced proteasome abundance and aberrations in ELVAs distribution and activity were found, providing a mechanistic explanation for the diminished developmental competence aged oocytes. Taken together, our findings elucidate the critical function of proteasome-regulated proteostasis in oocyte quality control during OET and reproductive aging in females.","dates":{"publication":"2026/04/27"},"accession":"GSE293047","cross_references":{"GSM":["GSM8874510","GSM8874511","GSM8874500","GSM8874505","GSM8874506","GSM8874507","GSM8874508","GSM8874512","GSM8874501","GSM8874502","GSM8874513","GSM8874514","GSM8874503","GSM8874515","GSM8874504","GSM8874509"],"GPL":["24247"],"GSE":["293047"],"taxon":["Mus musculus"]}}