GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293572/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293572GEOGSEfalseCAF drives spheroid formation and pleural dissemination in NSCLCPleural dissemination in non-small cell lung cancer (NSCLC) refers to the direct spread of cancer cells from the primary tumor site to the extrapulmonary thoracic cavity, where they colonize the pleura. This study aimed to investigate the impact of cancer-associated fibroblasts (CAFs) on the development of pleural dissemination in NSCLC. Through in vitro co-culture and in vivo pleural dissemination models, we identified that CAFs promote cancer cell migration, anchorage-independent growth, adhesion, invasion, and spheroid formation of cancer cells, leading to the development of pleural dissemination. RNA sequencing analysis revealed that CAFs upregulated the expression of Cellular Communication Network Factor 1 (CCN1), a matricellular protein, in cancer cells.2026/04/02GSE293572GSM8885661GSM8885660GSM8885665GSM8885664GSM8885663GSM8885662GSM8885658GSM888565928038293572Homo sapiens