GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293592/primaryOK200Methylation profilingMus musculusMethylation profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293592GEOGSEfalseMaternal immune activation delays DNA methylation and physiological maturation of cortical excitatory neuronsElevated levels of maternal pro-inflammatory cytokines during gestation can disrupt offspring neural development, increasing the risk of neurodevelopmental disorders. We studied the effects of Poly(I:C)-induced maternal immune activation (PIC-MIA) during mid-gestation on the DNA methylation and transcriptome of developing cortical excitatory neurons. PIC-MIA disrupted the developmental regulation of synapse-related genes and of genes implicated in autism spectrum disorders. Genomic regions that gain or lose DNA methylation during normal development were altered following PIC-MIA, including neurodevelopmental transcription factor binding sites. The DNA methylation and transcriptional changes were consistent with a delay in excitatory neuron maturation. Whole-cell recordings showed that PIC-MIA preferentially altered the physiological development of layer 5 excitatory neurons. Taken together, present results suggest that alterations in the epigenome, through the disruption of circuit formation, may drive the long-term consequences of maternal infection during gestation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 musculus[42255505]